全文获取类型
收费全文 | 1396篇 |
免费 | 153篇 |
出版年
2018年 | 13篇 |
2017年 | 15篇 |
2016年 | 17篇 |
2015年 | 32篇 |
2014年 | 49篇 |
2013年 | 51篇 |
2012年 | 70篇 |
2011年 | 60篇 |
2010年 | 41篇 |
2009年 | 38篇 |
2008年 | 63篇 |
2007年 | 60篇 |
2006年 | 57篇 |
2005年 | 44篇 |
2004年 | 47篇 |
2003年 | 49篇 |
2002年 | 47篇 |
2001年 | 37篇 |
2000年 | 48篇 |
1999年 | 33篇 |
1998年 | 18篇 |
1997年 | 13篇 |
1996年 | 16篇 |
1995年 | 18篇 |
1994年 | 12篇 |
1992年 | 26篇 |
1991年 | 20篇 |
1990年 | 28篇 |
1989年 | 24篇 |
1988年 | 28篇 |
1987年 | 28篇 |
1986年 | 18篇 |
1985年 | 29篇 |
1984年 | 21篇 |
1983年 | 13篇 |
1982年 | 18篇 |
1981年 | 16篇 |
1980年 | 13篇 |
1979年 | 15篇 |
1977年 | 18篇 |
1975年 | 13篇 |
1974年 | 23篇 |
1973年 | 23篇 |
1972年 | 20篇 |
1971年 | 16篇 |
1970年 | 12篇 |
1969年 | 14篇 |
1968年 | 18篇 |
1967年 | 16篇 |
1966年 | 11篇 |
排序方式: 共有1549条查询结果,搜索用时 15 毫秒
21.
E. M. Holt I. E. Boyd C. J. Dewhurst J. Murray C. H. Naylor J. H. Smitham 《BMJ (Clinical research ed.)》1973,3(5870):39-43
Intrauterine transfusion was performed or attempted in 101 cases during a period of four and a half years. After exclusion of six cases, for reasons detailed in the text, 95 cases are considered in detail. Intrauterine transfusion was successfully performed in 93 of these, with a mean of 2·8 transfusions each. Forty-four babies (46·3%) survived and all but one are so far developing normally. 相似文献
22.
Species distributional limits may coincide with hard dispersal barriers or physiological thresholds along environmental gradients, but they may also be influenced by species interactions. We explore a number of models of interspecific interactions that lead to (sometimes abrupt) distribution limits in the presence and absence of environmental gradients. We find that gradients in competitive ability can lead to spatial segregation of competitors into distinct ranges, but that spatial movement tends to broaden the region of sympatry between the two species, and that Allee effects tend to sharpen these boundaries. We generalize these simple models to include metapopulation dynamics and other types of interactions including predator–prey and host–parasite interactions. We derive conditions for range limits in each case. We also consider models that include coevolution and gene flow and find that character displacement along environmental gradients can lead to stable parapatric distributions. We conclude that it is essential to consider coevolved species interactions as a potential mechanism limiting species distributions, particularly when barriers to dispersal are weak and environmental gradients are gradual. 相似文献
23.
The number of high affinity [3H]GDP binding sites in brown adipose tissue mitochondria is normal in obese ( f a / f a ) rats in contrast to the reduced number of low affinity GDP binding sites. Adrenalectomy corrected the loss of low affinity binding sites in fa/fa rats but had no effect on the number of high affinity sites in either lean or obese rats. Equilibrium dialysis was used to show the presence of both high and low affinity binding sites on the purified 32 kdalton protein. 相似文献
24.
25.
Effects of peptidoglycans from periodontal pathogens on selected biological activities of CD-1 murine peritoneal macrophages 总被引:2,自引:0,他引:2
Resident CD-1 murine peritoneal macrophages were exposed to various concentrations of purified peptidoglycan isolated from members of the genera Bacteroides, Eikenella, and Actinomyces. Macrophage viability, the release of lysozyme, acid phosphatase, and prostaglandins E1 and E2 were assayed as a function of peptidoglycan concentration and time. Macrophages responded as a function of peptidoglycan concentration with increased release of acid phosphatase and prostaglandins; all cells remained greater than 90% viable during the course of the experiments. However, concentrations of peptidoglycan greater than 50 micrograms/mL were toxic to the macrophages, while the peptidoglycan from B. capillus strain 925.08 and Actinomyces viscosus strain T14AV consumed complement by both the classical and the alternate pathways. Cellular lysozyme activity and phagocytosis of Saccharomyces cerevisiae were significantly reduced in the presence of peptidoglycan. When viewed by scanning electron microscopy, the activated macrophages were rounded, lacked distinct pseudopod extensions, and possessed an increased number of microvilli and plasma membrane associated vesicles. These morphological alterations occurred as early as 3 h. Transmission electron microscopy revealed the purified peptidoglycan to have been taken up into numerous phagosomes; however, even after 24 h incubation, it was only partially degraded. 相似文献
26.
27.
Mark J. Henderson Heather A. Baldwin Christopher A. Werley Stefano Boccardo Leslie R. Whitaker Xiaokang Yan Graham T. Holt Eric R. Schreiter Loren L. Looger Adam E. Cohen Douglas S. Kim Brandon K. Harvey 《PloS one》2015,10(10)
Endoplasmic reticulum calcium homeostasis is critical for cellular functions and is disrupted in diverse pathologies including neurodegeneration and cardiovascular disease. Owing to the high concentration of calcium within the ER, studying this subcellular compartment requires tools that are optimized for these conditions. To develop a single-fluorophore genetically encoded calcium indicator for this organelle, we targeted a low affinity variant of GCaMP3 to the ER lumen (GCaMPer (10.19)). A set of viral vectors was constructed to express GCaMPer in human neuroblastoma cells, rat primary cortical neurons, and human induced pluripotent stem cell-derived cardiomyocytes. We observed dynamic changes in GCaMPer (10.19) fluorescence in response to pharmacologic manipulations of the ER calcium store. Additionally, periodic calcium efflux from the ER was observed during spontaneous beating of cardiomyocytes. GCaMPer (10.19) has utility in imaging ER calcium in living cells and providing insight into luminal calcium dynamics under physiologic and pathologic states. 相似文献
28.
29.
30.
Shalindra Ranasinghe Renu Wickremasinghe Sanjeeva Hulangamuwa Ganga Sirimanna Nandimithra Opathella Rhaiza DC Maingon Vishvanath Chandrasekharan 《Memórias do Instituto Oswaldo Cruz》2015,110(8):1017-1023
Leishmania donovani is the known causative agent of both cutaneous
(CL) and visceral leishmaniasis in Sri Lanka. CL is considered to be under-reported
partly due to relatively poor sensitivity and specificity of microscopic diagnosis.
We compared robustness of three previously described polymerase chain reaction (PCR)
based methods to detectLeishmania DNA in 38 punch biopsy samples
from patients presented with suspected lesions in 2010. Both,
Leishmaniagenus-specific JW11/JW12 KDNA and LITSR/L5.8S internal
transcribed spacer (ITS)1 PCR assays detected 92% (35/38) of the samples whereas a
KDNA assay specific forL. donovani (LdF/LdR) detected only 71%
(27/38) of samples. All positive samples showed a L. donovani
banding pattern upon HaeIII ITS1 PCR-restriction fragment length polymorphism
analysis. PCR assay specificity was evaluated in samples containing
Mycobacterium tuberculosis, Mycobacterium
leprae, and human DNA, and there was no cross-amplification in JW11/JW12
and LITSR/L5.8S PCR assays. The LdF/LdR PCR assay did not amplify M.
leprae or human DNA although 500 bp and 700 bp bands were observed in
M. tuberculosis samples. In conclusion, it was successfully shown
in this study that it is possible to diagnose Sri Lankan CL with high accuracy, to
genus and species identification, using Leishmania DNA PCR
assays. 相似文献